The Nanopore Reader 100 KHz is a compact and portable read-out device with reusable flow-cells for ultra low noise recordings of solid state nanopores and biological pores.
Solid State Nanopore
The Nanopore Reader can be used for experimental activities such as lipid bilayer measurements, DNA translocation and single molecule detection.
If combined with the specific flowcell, it is suitable for protein study, disease marker detection, DNA mapping, and nanoparticle analysis.
Miniaturized – Handheld instruments vs. bulky setups
Ready to use – Tools free, just insert the flow cell in the device
High quality performances – low noise measurements
Affordable – We enable technologies for everyone!
Exemplary current trace of KCVnts channel recorded at -60mV in symmetrical 1M KCl, PH 7 (with 1,25 KHz sampling rate and 625 Hz bandwidth). The channel protein was translated in vitro into NDs (nanodiscs) with DMPC membranes. The purified NDs in dilution with imidazole were directly administered to the bilayer formed by painting DPhPc over our 150 µm diameter BLMchip designed for biological pores. The open and closed level of the channel are indicated. The top panel shows the signal of the membrane at -60mV before the insertion of the channel protein. KCVnts is gift from Prof. G. Thiel (TU-Darmstadt University).
Exemplary current trace of a single α-Hemolysin channel insertion recorded at 5 KHz sampling rate (and 2,5 KHz bandwidth), applying a membrane potential of 100mV, in symmetrical 1M KCl, PH 7. PEG1000 molecules (already present in solution) translocate through the α-Hemolysin channel giving rise to negative individual blockades. The channel protein was directly added in solution and auto-assembled into the bilayers made of DPhPc.
A bilayer membrane was formed by painting DPHPC lipids (10mg/ml in n-octane) over the 100uM hole of the BLM-chip filled with asymmetrical HCl solutions. When a stable membrane was obtained, a small amount of gramicidin was added to the recording solutions. After some minutes, the formation of gramicidin dimers allowed the flow of H+ ions across the membrane.
Representative single channel currents of wt KcsA reconstituted in liposomes and incorporated in POPE:POPG lipid bilayers (w:w, 3:1). Data courtesy of Prof. M. F. Tsai (University of Colorado School of Medicine, USA).
Representative current trace showing single channel activity of chloride intracellular channel 1 (CLIC1) reconstituted in DPhPC lipid bilayer membranes painted over a 150 µm hole. Data courtesy of Prof. M. Mazzanti (University of Milan, Italy).
dsDNA fragment translocation data obtained with a 17-nm-diameter SiN x pore at +200 mV,
1M KCl (10 mM Tris buffer, 1 mM EDTA and pH 8.0) for (a) 15 kbp, (b) 1000 bp, and (c)
400 bp dsDNA, and corresponding event duration histograms measured at 100 kHz
bandwidth. Red curves are exponential fits to obtain the characteristic dwell times.
Modified from Niedzwiecki et al Rev Sci Instrum. 2020 Mar 1;91(3):031301
350 nm sized polystyrene nanoparticle translocation obtained at 420 mV with a laser
drilled 1µm pore in Polyimide layer. Data were acquired at 50 kHz SR, 25 kHz bandwidth.
PEG-25 (10 µM) molecules translocation through a single α-Hemolysin nanopore recorded at -100 mV, 20kHz SR (10 kHz BW). The protein was inserted into a DPhPC-made lipid bilayer membrane painted into our BLMchip embedding a 150 µm sized hole. The recording solution contained 3M KCl, 20mM TRIS, PH 8.
- Open input (RMS) noise (Voltage range ±700mV) : 0.06 pA rms @ 625Hz; 0.3 pA rms @ 10 kHz; 2.4 pA rms @ 100 kHz
- Open input (RMS) noise (Voltage range ±2000mV) : 0.08 pA rms @ 1kHz; 0.42 pA rms @ 10 kHz; 3.7 pA rms @ 100 kHz
- Current ranges: ±200pA (Gain 2.25GΩ), ±2nA (Gain 225MΩ), ±20nA (Gain 22.5MΩ), ±200nA (Gain 2.25MΩ)
- Voltage hold ranges: ±700mV (ultra low noise); ±2000mV (low noise)
- Parametric voltage protocols
- Max sampling rate: 200 ksps
- Selectable x4 oversampling (max final sampling rate 800 ksps)
- Available bandwidth between 62.5 Hz to 100 kHz
- Auto electrodes voltage offset fine compensation
- Continuous Capacitance and Resistance estimation
- USB powered
- Size & Weight: 101 x 44 x 18 mm, 140 g
Nanopore Reader Guides
- Connection diagram
- Get started with the model cell
- Ultra low noise modality
- eNPR voltage protocols
- How to use the eNPR adaptor to connect external recording chambers
Solid State Nanopore Guides
Biological Nanopore Guides
- Kukovetz K et al., A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. Viruses. 2020 Sep 29;12(10):1107.
- Niedzwiecki DJ et al., Detection of single analyte and environmental samples with silicon nitride nanopores: Antarctic dirt particulates and DNA in artificial seawater. Rev Sci Instrum. 2020 Mar 1;91(3):031301.
- Niedzwiecki DJ et al., DNA fragment translocation in artificial sea water through nanopores using a portable mini reader and flow-cell. Poster presented at BPS meeting.
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