BLM kit

A compact and complete educational kit for accurate electrophysiological recordings.
Especially designed for teaching activities. The kit includes all the tools for fast screening of ion-channels functional activity in lipid environments.

Why to choose the BLM kit?

Miniaturized – Without bulky setup you can start doing experiments on your desk

User friendly – Stable membranes can be easily formed to study ion channels or toxin properties

Teaching activities – Especially designed to enable students to learn electrophysiology basis on lipid bilayer properties, membrane protein study and ion channel analysis

What is inside?

The kit includes:

  • eONE Amplifier (Light, Plus or High Current Version)
  • Faraday Cage (16x10x5 cm)
  • EDR Software
  • Tools: 6 Teflon or Polyimide Membranes (aperture size: 50, 75, 100, 150, 200 μm), Paint Brush, Ag wires, Electrode connectors
  • Recording chamber: BLM Cuvette_MM

BLM Cuvette_MM

The new cuvette is engineered to reduce the training time needed to paint high-quality lipid membranes and prevent leaks caused by assembly errors. Its geometry encourages vertical membrane growth, reducing membrane width by ensuring compatibility with techniques like Montal-Müller painting and the pseudo-air bubble method. These methods produce superior quality membranes in record time compared to tools-involving approaches (e.g. paint brush method). Additionally, the use of silicon grease to seal Delrin parts with Kapton foil drastically reduces the risk of chamber leakage, eliminating the inconvenience of frequent silicone seal replacements.

Lipid Bilayer Formation: Vertical

Chambers Volume: 1 mL

Septa aperture sizes: 50, 75, 100, 150 or 200 µm

How to perform a Lipid Bilayer experiment using the BLM kit?

Septum thickness 50 µm 50 µm 50 µm 50 µm
Aperture Ø 50 µm 75 µm 100 µm 150 µm
Typical BLM capacitance (pF) 30 – 40 50 – 60 70 – 90 120 – 150

 

*Membranes were painted with Montal-Müller technique using DPhPC lipids

Custom device development

Let us know how we can help you design the tools and software you need.