A complete accessory kit for the eONE amplifier, designed for teaching and fast screening of ion channel functional activity in lipid environments. Includes all the essential tools for accurate electrophysiological recordings.
Why to choose the BLM kit?
Miniaturized – Without bulky setup you can start doing experiments on your desk
User friendly – Stable membranes can be easily formed to study ion channels or toxin properties
Teaching activities – Especially designed to enable students to learn electrophysiology basis on lipid bilayer properties, membrane protein study and ion channel analysis
What is inside?
The kit is designed for the eONE Amplifier (Light, Plus or High Current Version) that comes with EDR Software.
The kit consists of:
- Faraday Cage (16x10x5 cm)
- Amplifier Holder
- Recording chamber: BLM Cuvette_MM
- Tools: 6 Teflon or Polyimide Membranes (aperture size Polyimide: 50, 75, 100, 150, 200 μm; aperture size Teflon: 50, 80, 100), Paint Brush, Ag wires, Electrode connectors
BLM Cuvette_MM
The new cuvette is engineered to reduce the training time needed to paint high-quality lipid membranes and prevent leaks caused by assembly errors. Its geometry encourages vertical membrane growth, reducing membrane width by ensuring compatibility with techniques like Montal-Müller painting and the pseudo-air bubble method. These methods produce superior quality membranes in record time compared to tools-involving approaches (e.g. paint brush method). Additionally, the use of silicon grease to seal Delrin parts with polyimide foil drastically reduces the risk of chamber leakage, eliminating the inconvenience of frequent silicone seal replacements.
Lipid Bilayer Formation: Vertical
Chambers Volume: 1 mL
Aperture size Polyimide: 50, 75, 100, 150 or 200 µm
Aperture sizes Teflon: 50, 80, 100 µm
How to perform a Lipid Bilayer experiment using the BLM kit?
| Septum thickness | 50 µm | 50 µm | 50 µm | 50 µm |
| Aperture Ø | 50 µm | 75 µm | 100 µm | 150 µm |
| Typical BLM capacitance (pF) | 30 – 40 | 50 – 60 | 70 – 90 | 120 – 150 |
*Membranes were painted with Montal-Müller technique using DPhPC lipids
Custom device development
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