Nanopore Reader 100 KHz

A compact and portable read-out device with reusable flow-cells for ultra low noise recordings of solid state nanopores and biological pores. It is suitable for experimental activities such as lipid bilayer measurements, DNA translocation and single molecule detection.

Same instrument, two applications

If combined with the specific flowcell, the Nanopore Reader 100 kHz can be used with both biological pores and solid state nanopores. Just ask your desired Flow Cell!

Biological pores

Biological nanopores are purified nanosized pore forming proteins inserted in a membrane of choice; mostly planar lipid membranes housed inside an electrochemical chamber. By monitoring ion currents and forces as molecules pass through a biological nanpore, it is possible to investigate a wide range of phenomena involving DNA and RNA.

Use Cases

Solid State pores

Solid State Nanopores Application

Artificial solid-state nanopores are nanosized pores fabricated in insulating membranes. By monitoring ion currents and forces as molecules pass through a solid-state nanopore, it is possible to investigate a wide range of phenomena involving DNA, RNA and proteins.

Use Cases

Why to choose the eNPR?

Miniaturized – Handheld instruments vs. bulky setups

Ready to use – Tools free, just insert the flow cell in the device

High quality performances – low noise measurements

Affordable – We enable technologies for everyone!

  • Open input (RMS) noise (Voltage range ±700mV) : 0.06 pA rms @ 625Hz; 0.3 pA rms @ 10 kHz; 2.4 pA rms @ 100 kHz
  • Open input (RMS) noise (Voltage range ±2000mV) : 0.08 pA rms @ 1kHz; 0.42 pA rms @ 10 kHz; 3.7 pA rms @ 100 kHz
  • Current ranges: ±200pA (Gain 2.25GΩ), ±2nA (Gain 225MΩ), ±20nA (Gain 22.5MΩ), ±200nA (Gain 2.25MΩ)
  • Voltage hold ranges: ±700mV (ultra low noise); ±2000mV (low noise)
  • Parametric voltage protocols
  • Max sampling rate: 200 ksps
  • Selectable x4 oversampling (max final sampling rate 800 ksps)
  • Available bandwidth between 62.5 Hz to 100 kHz
  • Auto electrodes voltage offset fine compensation
  • Continuous Capacitance and Resistance estimation
  • USB powered
  • Size & Weight: 101 x 44 x 18 mm, 140 g

 

Click here to watch a video-description of the technical specification of eNPR amplifier and EDR3 software together with some representative applications and data

Bilayer Lipid Membrane Chip (Flow Cell for Biological Nanopores) – The Flowcell is made by a layer of Polymide 12.5 μm thick and it hosts the micro hole for BLM formation that is available in two different sizes (100 μm, 150 μm). Both chambers are accessible and can be filled with low volumes (60 μl).

  • Reusable Flowcells: depending on your application you can use multiple times a single flowcell.
  • Typical membrane capacitance in the BLMchip:   20-50 pF in BLMchip 100 µm | 50-110 pF in BLMchip 150 µm

Flow Cell (for Solid State Nanopores) – The nanopore containing chip fits in a 5×5 mm² cavity and is secured with silicone rubber gaskets. Both chambers are accessible and can be filled with low volumes (10 μl – 60 μl of electrolyte solution). The nanopore chip can be rapidly changed without tools and the flow cell is reusable.

  • Flowcell materials: Standard PMMA, Delrin, or Teflon
  • Nanopore Chip not included
  • Chip thickness: 200 µm

 

Custom device development

With our custom ASIC design technology we can help you configure specific tools and solutions for your applications.

Let us know how we can help you design the tools and software you need.