A compact and portable read-out device with reusable flow-cells for ultra low noise recordings of solid state nanopores and biological pores. It is suitable for experimental activities such as lipid bilayer measurements, DNA translocation and single molecule detection.
Nanopore Reader 100 KHz

Same instrument, two applications

If combined with the specific flowcell, the Nanopore Reader 100 kHz can be used with both biological pores and solid state nanopores. Just ask your desired Flow Cell!
Biological pores
Biological nanopores are purified nanosized pore forming proteins inserted in a membrane of choice; mostly planar lipid membranes housed inside an electrochemical chamber. By monitoring ion currents and forces as molecules pass through a biological nanpore, it is possible to investigate a wide range of phenomena involving DNA and RNA.

Use Cases
Exemplary current trace of KCVnts channel recorded at -60mV in symmetrical 1M KCl, PH 7 (with 1,25 KHz sampling rate and 625 Hz bandwidth). The channel protein was translated in vitro into NDs (nanodiscs) with DMPC membranes. The purified NDs in dilution with imidazole were directly administered to the bilayer formed by painting DPhPc over our 150 µm diameter BLMchip designed for biological pores. The open and closed level of the channel are indicated. The top panel shows the signal of the membrane at -60mV before the insertion of the channel protein. – Prof. G. Thiel (TU-Darmstadt University).
Exemplary current trace of a single α-Hemolysin channel insertion recorded at 5 KHz sampling rate (and 2,5 KHz bandwidth), applying a membrane potential of 100mV, in symmetrical 1M KCl, PH 7. PEG1000 molecules (already present in solution) translocate through the α-Hemolysin channel giving rise to negative individual blockades. The channel protein was directly added in solution and auto-assembled into the bilayers made of DPhPc.
Exemplary current trace of gramicidin D single channel. A bilayer membrane was formed by painting DPHPC lipids (10mg/ml in n-octane) over the 100uM hole of the BLM-chip filled with asymmetrical HCl solutions. When a stable membrane was obtained, a small amount of gramicidin was added to the recording solutions. After some minutes, the formation of gramicidin dimers allowed the flow of H+ ions across the membrane.
Representative single channel currents of wt KcsA reconstituted in liposomes and incorporated in POPE:POPG lipid bilayers (w:w, 3:1).
– Data courtesy of Prof. M. F. Tsai (University of Colorado School of Medicine, USA).
Representative current trace showing single channel activity of chloride intracellular channel 1 (CLIC1) reconstituted in DPhPC lipid bilayer membranes painted over a 150 µm hole.
– Data courtesy of Prof. M. Mazzanti (University of Milan, Italy).
PEG-25 (10 µM) molecules translocation through a single α-Hemolysin nanopore recorded at -100 mV, 20kHz SR (10 kHz BW). The protein was inserted into a DPhPC-made lipid bilayer membrane painted into our BLMchip embedding a 150 µm sized hole. The recording solution contained 3M KCl, 20mM TRIS, PH 8.
Perform your Lipid Bilayer Experiment!
Solid State pores

Artificial solid-state nanopores are nanosized pores fabricated in insulating membranes. By monitoring ion currents and forces as molecules pass through a solid-state nanopore, it is possible to investigate a wide range of phenomena involving DNA, RNA and proteins.
Use Cases
dsDNA fragment translocation data obtained with a 17-nm-diameter SiN x pore at +200 mV, 1M KCl (10 mM Tris buffer, 1 mM EDTA and pH 8.0) for (a) 15 kbp, (b) 1000 bp, and (c) 400 bp dsDNA, and corresponding event duration histograms measured at 100 kHz bandwidth. Red curves are exponential fits to obtain the characteristic dwell times.
– Data courtesy from Niedzwiecki et al Rev Sci Instrum. 2020 Mar 1;91(3):031301
Exemplary current trace of 200 and 350 nm sized polystyrene nanoparticles translocating trough a laser drilled Polymide-made 1µm pore. The signal was sampled at 20 kHz SR by applying + 600 mV of bias voltage. Two distinct population are visible, corresponding to the 200 and 350 nm sized particles.
Why to choose the eNPR?

Miniaturized – Handheld instruments vs. bulky setups

Ready to use – Tools free, just insert the flow cell in the device

High quality performances – low noise measurements

Affordable – We enable technologies for everyone!
- Open input (RMS) noise (Voltage range ±700mV) : 0.06 pA rms @ 625Hz; 0.3 pA rms @ 10 kHz; 2.4 pA rms @ 100 kHz
- Open input (RMS) noise (Voltage range ±2000mV) : 0.08 pA rms @ 1kHz; 0.42 pA rms @ 10 kHz; 3.7 pA rms @ 100 kHz
- Current ranges: ±200pA (Gain 2.25GΩ), ±2nA (Gain 225MΩ), ±20nA (Gain 22.5MΩ), ±200nA (Gain 2.25MΩ)
- Voltage hold ranges: ±700mV (ultra low noise); ±2000mV (low noise)
- Parametric voltage protocols
- Max sampling rate: 200 ksps
- Selectable x4 oversampling (max final sampling rate 800 ksps)
- Available bandwidth between 62.5 Hz to 100 kHz
- Auto electrodes voltage offset fine compensation
- Continuous Capacitance and Resistance estimation
- USB powered
- Size & Weight: 101 x 44 x 18 mm, 140 g
Bilayer Lipid Membrane Chip (Flow Cell for Biological Nanopores) – The Flowcell is made by a layer of Polymide 12.5 μm thick and it hosts the micro hole for BLM formation that is available in two different sizes (100 μm, 150 μm). Both chambers are accessible and can be filled with low volumes (60 μl).
- Reusable Flowcells: depending on your application you can use multiple times a single flowcell.
- Typical membrane capacitance in the BLMchip: 20-50 pF in BLMchip 100 µm | 50-110 pF in BLMchip 150 µm
Flow Cell (for Solid State Nanopores) – The nanopore containing chip fits in a 5×5 mm² cavity and is secured with silicone rubber gaskets. Both chambers are accessible and can be filled with low volumes (10 μl – 60 μl of electrolyte solution). The nanopore chip can be rapidly changed without tools and the flow cell is reusable.
- Flowcell materials: Standard PMMA, Delrin, or Teflon
- Nanopore Chip not included
- Chip thickness: 200 µm
Nanopore Reader Guides
- Connection diagram
- Get started with the model cell
- Ultra low noise modality
- eNPR voltage protocols
- How to use the eNPR adaptor to connect external recording chambers
Solid State Nanopore Guides
Biological Nanopore Guides
Biological Nanopores
Solid State Nanopores
- High Accuracy Protein Identification: Fusion of Solid-State Nanopore Sensing and Machine Learning, Dutt, S., Shao, H., Karawdeniya, B., Bandara, Y. M. N. D. Y., Daskalaki, E., Suominen, H., Kluth, P., Small Methods 2023, 2300676
- Ultrathin, High-Lifetime Silicon Nitride Membranes for Nanopore Sensing, Dutt S et al. Anal. Chem., 2023
- Large-scale production of polyimide micropore-based flow cells for detecting nano-sized particles in fluids, Salehirozveh et al., RSC Adv., 2023, 13, 873-880
- Nanopores: a versatile tool to study protein dynamics, Schmid S, Dekker C., Essays Biochem., 2021
- Detection of single analyte and environmental samples with silicon nitride nanopores: Antarctic dirt particulates and DNA in artificial seawater., Niedzwiecki DJ et al., Rev Sci Instrum., 2020 Mar 1;91(3):031301.
- DNA fragment translocation in artificial sea water through nanopores using a portable mini reader and flow-cell., Niedzwiecki DJ et al., Poster presented at BPS meeting.
📣 New Release EDR4: Check compatibility with your device HERE.

Custom device development
With our custom ASIC design technology we can help you configure specific tools and solutions for your applications.
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