Nanopore Reader HC

Handheld and fully integrated 1-channel high-current amplifier for low-noise measurements. Suitable for large currents generated in solid-state nanopores.

Download Brochure

Request Quote

Flow cells for Solid-State Pores

Nanopore Flow cell

A microfluidic system serving as the interface between the nanopore chip and the current reader. The nanopore chip can be easily changed without tools and the flow cell is reusable.

Nanopore Chip: Vertical

Chambers Volume: 10 µl min – 60 µl max

Flow cell Materials: PMMA or Teflon

Replaceable External Silver Wires

Nanopore Chip not Included, buy it from Norcada

Nanopore Chip Compatibility: Square 4×4-5×5 mm², 200-700 µm thick and round 3×3 mm², 200 µm thick

Reusable flow cell

Micropore Flowcell

A microfluidic system serving as the interface between the micro-aperture and the current reader. The pore dimensions are customisable, starting from 1 µm and larger, and the flow cell is disposable.

Micro-aperture: Horizontal

Chambers Volume: 20 µl bottom compartment- 80 µl top compartment

Flow cell Materials: Polyethylene with Polyimide foil

Integrated Printed Ag/AgCl electrodes

Customisable micro-aperture drilled in polyimide foil

Disposable flow cell

Use Cases

Translocation of two Nanoparticle Populations using a Micropore Flow cell

Representative 10-second recording of resistive pulses measured in the micropore flow cell after the addition of polystyrene nanoparticles, 100 and 350 nm in size, in a 1:1 mixture (concentration: E12 particles/mL in 1X PBS). The two apertures providing access to the bottom compartment (see panel A) were clogged after the addition of the sample in order to apply a pressure.
Histogram analysis of the peak amplitude shows two clear distinct populations, namely the 100 and the 300 nm particles. Inset shows a blow-up of two representative events.

Translocation of AAVs using a nanopore chip mounted in Elements flow cell

Translocation of purified AAV9 capsids through a 60 nm nanopore measured at 120 mV in 2 M LiCl. The histogram shows the number of events versus the peak amplitudes over a 300-second recording. The 5×5 mm chip housing the 60 nm pore was mounted in our flow cell for solid-state nanopores.

Features

Open input (RMS) noise (Voltage range ±700mV, Current Range ±200nA) : 9 pA rms @ 25 kHz; 32 pA rms @ 100 kHz
Open input (RMS) noise (Voltage range ±2000mV, Current Range ±200nA) : 15 pA rms @ 25kHz; 45 pA rms @ 100 kHz
Current ranges: ±200 nA; ±4 µA
Voltage hold ranges: ±700mV (ultra low noise); ±2000mV (low noise)
Parametric voltage protocols
Max sampling rate: 200 ksps
Available bandwidth between 625 Hz to 100 kHz
Auto electrodes voltage offset fine compensation
Continuous Capacitance and Resistance estimation
USB powered
Size & Weight: 101 x 44 x 18 mm, 140 g