Nanion Technologies GmbH

Jan 16

Nanion Technologies GmbH

Thanks to Nanion GmbH for the measurements done by A. Marabelli and M. Kreir

Single channel recordings of different ion channels reconstituted into planar lipid bilayers formed using GUVs (giant unilamellar vesicles) and the Nanion's Port-a-Patch. The GUVs were formed by electroformation using 10 mM DPhPC (diphytanoyl phosphatidylcholine) lipid and 1 mM DOPA (Dioleyl glycero phosphatidic acid) lipid. Signals were acquired at room temperature using a sampling rate of 10KHz. Depending on the ion channel, recordings were performed in different buffer solutions and applying different transmembrane potentials, respectively:
Kv1.3 potassium channel (protein concentration 30ug/ml). Buffer solution: 200 mM KCl, 10 mM HEPES, pH 7. +80 mV voltage applied.
CarO channel (protein concentration 5ug/ml). Buffer solution: 200 mM KCl, 10 mM HEPES, pH 5.
TrPA1 receptor. Buffer solution: 140 mM NaCl, 4 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 5 mM D-Glucose monohydrate, 10 mM Hepes /NaOH pH 7.4. Internal solution: 50 mM KCl, 10 mM NaCl, 60 mM K-Fluoride, 20 mM EGTA, 10 mM Hepes /KOH, pH 7.2. Cinnamon (1mM) was dissolved in DMSO (final 0.1%). +50 mV voltage applied.